We took soybeans as experimental substance to study how the 532nm laser with different power density and irradiation
time affected the pullulation ratio of the soybeans, average height of seedlings and the isoflavone content of seedlings'
cotyledon and laminae. The mechanism that laser pretreatment of soybean seeds could increase the isoflavone content of
the seedlings was discussed in such both aspects as the efficiency of the photosynthesis and the activity of a
phenylalanine ammonia-lyase (PAL) as an initial enzyme for synthesizing the isoflavone. The results showed that after
the soybean seeds were pretreated by laser, the activity of the PAL and the resultants of the photosynthesis such as the
sugar of dissolubility, the sucrose, and the amylum all increased with the soybean seeds irradiated by laser in which the
effect on the soybean seeds pretreated by 15mW/mm2 laser for 5 min was the most obvious. As a result, the
photosynthesis efficiency of the soybean seedlings increased after being pretreated by laser, which might offer the
foundation for accumulating a large amount of isoflavone.
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