KEYWORDS: Digital holography, Red blood cells, Holography, Biological imaging, Optical microscopy, Metrology, Holography applications, Microscopy, 3D metrology, 3D image processing
Imaging and measuring moving objects behind translucide layers is of high interest in numerous applications, in various fields. We developed in this study a method using digital holographic microscopy for that purpose, with a partial spatial coherence illumination. The presented study includes numeric simulations for the assessment of the measurement accuracy, as a function of both the object and the translucide layer parameters. Experiments are performed with the in vitro analysis of human red blood cells moving behind an edothelial cell layer. Such a configuration mimics the real blood vessel, which is of crucial importance for a better understanding of the behaviour of the blood cells in blood circulation.
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