As an alternative imaging technique to conventional IR microscopy, a mid-infrared photothermal microscopy has been suggested to achieve spatial resolution at the submicrometer level and the inherent chemical contrast upon vibrational excitation. It also has substantial potential for real-time imaging of live organisms to observe the cellular dynamics without photodamage or photobleaching of fluorescent labels. We performed real-time imaging of oligodendrocytes to investigate cellular dynamics and observed a photothermal contrast associated with traveling protein complexes on an axon of live neutrons. We anticipate that mid-infrared photothermal imaging will be of great use for gaining insights into the field of biophysical science, especially with regard to cellular dynamics and functions.
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