Radiation therapy (RT) is a standard treatment for ~50% of cancers worldwide. The major goal in RT is to maximize tumor cell kill while minimizing radiation-induced damage to healthy tissue. However, several fundamental questions remain unanswered related to the radiation-matter interactions, and the ensuing radiobiological responses in cells and tissue. Stimulated Raman scattering (SRS) microscopy is a promising technology for imaging the heterogeneous metabolic responses in cells exposed to ionizing radiation. In this work, we demonstrate SRS imaging of endogenous macromolecules such as lipids and proteins in the C-H stretching region (2800 to 3100 cm-1) in MCF-7 breast cancer cells in vitro. We compare the response of control (unirradiated) cells and cells irradiated at 30 Gy (120 kVp x-rays), at three time points of 24, 48, and 72 hours post exposure to ionizing radiation, and find significant changes between irradiated and control MCF-7 cells over time.
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