Trichomoniasis is one of the most common non-viral sexually transmitted infection caused by a parasite called Trichomonas vaginalis. Currently, there is no timely, cost-efficient diagnostic test exist for Trichomoniasis. We present a lensless optofluidic imaging technique for label-free point-of-care detection of motile parasites in bodily fluids. With this setup we are able to achieve a FOV of ~2 mm2 at a frame rate of 30 fps and can see ~200 μl of sample in one image. Trichomonas vaginalis is identifiable through simple morphological features. Contrary to common practice of minimizing the distance between the sample and the sensor to maximize the resolution, we demonstrated that a greater distance is more advantageous by using the parasites natural ability to focus light as a contrast mechanism. This technique uses a low-cost high frame rate CMOS detector, which enables high throughput operation with requires minimal sample preparation, making it a promising solution to the rapid diagnosis of Trichomoniasis.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.