Ms. Linda I. Rivera Profile

Linda I. Rivera

at Rensselaer Polytechnic Institute

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 17 November 2008 Paper

Multi-worm tracking using superposition of merit functions

Linda Ivonne Rivera, Benjamin Potsaid, John Ting-Yung Wen

Proceedings Volume 7266, 72661C (2008) https://doi.org/10.1117/12.816468

KEYWORDS: Detection and tracking algorithms, Mirrors, Head, Algorithm development, Imaging systems, Optical microscopes, Motion controllers, Computed tomography, Image segmentation, Optical tracking

Read Abstract +

Traditional solutions for long term imaging of living small biological specimens and microorganisms lack efficiency due to computationally expensive algorithms, and field of view limitations in optical microscopes. This paper describes a new algorithm that allows for real time tracking of multiple 1mm nematodes called Caenorhabditis elegans with a novel optical microscope design called the Adaptive Scanning Optical Microscope (ASOM), developed at the Center for Automation Technologies and Systems (CATS). Based on the real time experimentation, an improved algorithm to track multiple worms in the presence of entanglements is generated. The stages of this development start with an enhanced digital motion controller for the ASOM high speed scanning mirror to suppress undesired vibrations that limit the system capacity to track multiple organisms. The second phase is the integration of the ASOM apparatus, the high speed motion control, and a base tracking algorithm, all which allows for rapid image acquisition to track multiple C. elegans in real time. The base algorithm was developed at CATS and has been proven to track a single C. elegans in real time. Results demonstrating the efficacy of the complete system are presented. Lastly, an enhanced tracking algorithm is described that shows improved accuracy and robustness in tracking worms even when they become entangled. Taking into account the unique ASOM design, individual segments of the worm are tracked throughout an image sequence, and a mosaic pattern covering the entire worm is subsequently created. The algorithm takes advantage of geometric and dynamic knowledge of the C. elegans such as size, and movement patterns. The enhanced algorithm is tested on previously recorded footage. Simulated tracking experiments also illustrate the effectiveness of the enhanced algorithm and are presented.

Proceedings Article | 9 February 2007 Paper

Adaptive scanning optical microscope (ASOM): large field of view and high resolution imaging using a MEMS deformable mirror

Benjamin Potsaid, Linda Ivonne Rivera, John Ting-Yung Wen

Proceedings Volume 6467, 646706 (2007) https://doi.org/10.1117/12.700538

KEYWORDS: Deformable mirrors, Mirrors, Actuators, Image quality, Cameras, Microscopes, Lens design, Scanners, Microelectromechanical systems, Adaptive optics

Read Abstract +

For a wide range of applications in biology, medicine, and manufacturing, the small field of view associated with high resolution microscope systems poses a significant challenge in practice. This paper describes an optical microscope design, called the Adaptive Scanning Optical Microscope (ASOM), which uses a MEMS deformable mirror working with a specially designed scanning lens to achieve a greatly expanded field of view. Most adaptive optics systems (e.g. telescopes and ophthalmology instruments) are designed to achieve near ideal performance under nominal operating conditions and primarily use the adaptive optics element to compensate for a time varying disturbance to the wavefront that is external to the optical system. In contrast to this approach, the deformable mirror in the ASOM is an integral component of the optical system and the static (glass) optical elements have been specifically designed to match the shape correcting capabilities of the deformable mirror. Using a high speed steering mirror coordinated with the deformable mirror actuation voltages, the ASOM operates by scanning over the workspace and should achieve diffraction limited imaging over a region approximately two orders of magnitude larger in area than a traditional microscope design. With the rapid scanning capabilities allowed by the high speed steering mirror and by acquiring a complete image during each exposure, the ASOM offers advantages in dynamically reconfigurable and adaptable imaging with no agitation to the workspace. After describing the design and operating principle of the ASOM, we present results from a low cost ASOM prototype.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years