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Minqun Wang

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Proceedings Article | 17 May 2019 Paper

Wide field Raman super-resolution microscopy

Shuo Yan, Mingqun Wang, Hui Fang

Proceedings Volume 11170, 111703V (2019) https://doi.org/10.1117/12.2534015

KEYWORDS: Raman spectroscopy, Microscopy, Super resolution, Tunable filters, Nanoparticles, Super resolution microscopy, Raman scattering, Digital micromirror devices

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The appearance of fluorescent probes has greatly promoted the development of optical microscopy, while Raman scattering detection is a further progress attracting more applications of microscopy. However, the research on the wide-field detection and imaging of Raman signals is a challenging project in recent years. We propose a method combining super-resolution structured illumination microscopy (SIM) and the wide-field narrow-bandwidth filtering technique of the tunable filter, using a digital micro mirror device (DMD) to generate structured patterns, and a pair of filters whose cutoff frequency can change as the angle of incidence changes, which allows us to obtain super-resolution images of samples at specific Raman shift peaks. We build up a microscopy system using this method, perform imaging experiments on fluorescent beads of different emission wavelengths and achieve the expected results. In the 3T3 Cell marked with SERS beads imaging experiments, good results are also achieved. We hope that this technology can be applied to more occasions, such as dynamic imaging of biological structures.

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