Prof. Rong Wen Profile

Prof. Rong Wen

at Bascom Palmer Eye Institute

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Proceedings Article | 7 March 2019 Presentation

VIS-OCT based quantitative imaging of RPE Lipofuscin with customized A2E-PMMA reference target (Conference Presentation)

Zahra Nafar, Shuliang Jiao, Rong Wen

Proceedings Volume 10868, 1086805 (2019) https://doi.org/10.1117/12.2509005

KEYWORDS: Optical coherence tomography, Luminescence, Imaging systems, Optical filtering, Polymethylmethacrylate, Pathogens, Signal detection, Sensors, Optical properties, Imaging technologies

Read Abstract +

Lipofuscin is a fluorescent lipid/protein complex in the retinal pigment epithelium (RPE) that accumulates with age and contributes to the pathogenesis of retinal dystrophies. Therefore, quantification of lipofuscin, through fundus autofluorescence (FAF) imaging, is important in diagnosis and monitoring the progression of the diseases. However, the measured AF signal is affected by the excitation light intensity, the detector sensitivity, and the optical properties of the ocular media anterior to the RPE. We previously developed a simultaneous visible-light OCT and FAF imaging technology which is able to eliminate the pre-RPE attenuations. Further, we implemented two reference targets with known fluorescence efficiency and reflectivity in the retinal intermediate imaging plane to eliminate the effects of system fluctuations. However, we used an ND filter to reduce the fluorescence signal of the commercially available reference target to FAF level which brought the necessity of a separate reflection target for OCT. In this study, we introduce a customized reference standard target to serve as a common target for both AF and OCT. This reference target is composed of PMMA and synthesized A2E, the major fluorophore of lipofuscin. Homogenous mixtures of PMMA resist and A2E were prepared with an optimum concentration of A2E to avoid the necessity of the ND filter. A2E-PMMA solution was spin-coated on a silicon wafer. The fluorescent coating with 400nm thickness serves as the common FAF and OCT reference target. Using A2E in reference fabrication provides a similar excitation and emission spectrums to RPE lipofuscin, and thus a better reference for lipofuscin/A2E quantification.

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