Fluorescence imaging for surgical guidance is a proven modality that allows for visualization of fluorescent markers in numerous biological imaging applications. As the field continues to develop there is an urgent need for fluorescence-imaging standards and targets that enable system characterization, performance monitoring, and the development of analytical algorithms. 3D-printing technology has shown promise in providing biomimicking phantoms that allow simulation of realistic clinical conditions. Here, we present a comprehensive method for 3D printing fluorescent and tissue-equivalent material using photo-curable resins. We show the ability to print Indocyanine-green (ICG) equivalent material in complex shapes that would enable the evaluation of ICG-specific clinical systems. The method presented allows tuning of both the reduced scattering and absorption coefficients at multiple wavelengths, allowing for application-specific manufacturing of 3D-printed phantoms.
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