We propose and demonstrate a novel practical femtosecond laser source, which is to our knowledge, the smallest size
and potentially low cost. The innovation is the simple linear cavity design utilizing soliton mode-locking induced by
precise group velocity dispersion control. Average output power of 680 mW and pulse width of 162 fs were obtained at
around 1045 nm from a diode-pumped Yb3+:KY(WO4)2 laser. The pulse repetition rate was 2.8 GHz, leading to a pulse
peak power of 1.5 kW, which is sufficient for biomedical imaging. The laser module including the laser diode pump
system has a footprint of 8×4 cm2. Under automatic current control condition, stable operation of 3000-hour was
observed with an average power fluctuation of less than ±10 %. Furthermore, under automatic power control condition,
stable operation of 2000-hour was observed with an average power fluctuation of less than ±1 %. Using this laser
module, we successfully obtained clear two-photon fluorescence images of muntjac skin fibroblast cells stained with a
combination of fluorescent stains (Alexa Fluor 488 phalloidin and Alexa Flour 555 goat anti-mouse lgG).
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