Dr. Tieqiao Zhang Profile

Dr. Tieqiao Zhang

Research Fellow at National Institutes of Health

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Proceedings Article | 28 February 2006 Paper

Live cell imaging of the endocytosis and the intracellular trafficking of multifunctional lipid nanoparticles

Tieqiao Zhang, S. Narasimhan Danthi, Jianwu Xie, Dehong Hu, Peter Lu, King Li

Proceedings Volume 6095, 60950D (2006) https://doi.org/10.1117/12.645398

KEYWORDS: Nanoparticles, Particles, Luminescence, Microscopy, Live cell imaging, Medicine, Cancer, Oncology, Genetics, Diffusion

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Artificial lipid nanoparticles have drawn great attention due to their potential in medicine. Linked with targeting ligands, they can be used as probes and/or gene delivery vectors for specific types of target cells. Therefore, they are very promising agents in early detection, diagnosis and treatment of cancers and other genetic diseases. However, there are several barriers blocking the applications. Controlling the cellular uptake of the lipid nanoparticles is an important technical challenge to overcome. Understanding the mechanism of the endocytosis and the following intracellular trafficking is very important for improving the design and therefore the efficiency as a drug delivery system. By using fluorescence microscopy methods, we studied the endocytosis of lipid nanoparticles by live M21 cells. The movements of the nanoparticles inside the cell were quantitatively characterized and classified based on the diffusion behavior. The trajectories of nanoparticles movement over the cell membrane revealed hop-diffusion behavior prior to the endocytosis. Fast movement in large steps is observed in intracellular trafficking and is attributed to active movement along microtubule. These observations help to understand the mechanism of the endocytosis and the pathway of the particles in cells.

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