Yeka Aponte, a principal investigator at the NIH and an adjunct professor at Johns Hopkins Neuroscience, interviewed her mentor and colleague, Karl Deisseroth, research scientist and psychiatrist at Stanford School of Medicine, about his pioneering work in optogenetics and ongoing research.
Functional imaging in vivo has become a powerful tool for studying neuronal circuits during behavior. Here we use a microendoscopy system for deep-brain imaging from two distinct lateral hypothalamic neuron populations (i.e. LHPV and LHLEPR). By combining endomicroscopy, genetically encoded calcium indicators, optogenetics, and chemogenetics, we demonstrate that LHPV neurons modulate pain whereas LHLEPR neuronal activity encodes for appetitive behaviors in mice.
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