Dr. Zhe Guang Profile

Dr. Zhe Guang

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Author

Publications (19)

Proceedings Article | 13 March 2024 Presentation

Towards in-vivo, non-invasive, label-free, real-time 3D H&E imaging for clinical applications via 3D phase contrast from quantitative oblique back illumination microcopy (qOBM)

Francisco Robles, Richard Levenson, Tanishq Abraham, Paloma Casteleiro Costa, Zhe Guang, Caroline Filan

Proceedings Volume PC12852, PC128520Z (2024) https://doi.org/10.1117/12.3008577

KEYWORDS: 3D image processing, Biological imaging, Tissues, Stereoscopy, Real time imaging, Light sources and illumination, Biomedical applications, Phase contrast, In vivo imaging, 3D applications

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Proceedings Article | 12 March 2024 Presentation + Paper

Neural network assisted fast refractive index tomography in thick tissues with quantitative oblique back-illumination microscopy

Zhenmin Li, Paloma Casteleiro Costa, Zhe Guang, Francisco Robles

Proceedings Volume 12854, 128540J (2024) https://doi.org/10.1117/12.3003414

KEYWORDS: 3D image processing, Tomography, Image deconvolution, Neural networks, 3D image reconstruction, Biological samples, Microscopy, Deconvolution, Refractive index, Nervous system

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Proceedings Article | 12 March 2024 Presentation + Paper

Experimental assessment of the optical transfer function for quantitative oblique back-illumination microscopy

Zhenmin Li, Caroline Filan, Zhe Guang, Paloma Casteleiro Costa, Francisco Robles

Proceedings Volume 12854, 128540H (2024) https://doi.org/10.1117/12.3003347

KEYWORDS: Optical transfer functions, Monte Carlo methods, Tissues, Biological samples, Cameras, Scattering, Phase imaging, Objectives, Microscopy, Image restoration

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Proceedings Article | 12 March 2024 Poster + Paper

Multimodal fluorescence microscopy and 3D quantitative phase imaging/refractive index tomography in thick tissue samples

Zhenmin Li, Zhe Guang, Paloma Casteleiro Costa, Caroline Filan, Srinidhi Bharadwaj, Francisco Robles

Proceedings Volume 12852, 128520I (2024) https://doi.org/10.1117/12.3003242

KEYWORDS: Fluorescence microscopy, Fluorescence, Biological samples, Neuroimaging, Brain, 3D image processing, Imaging systems, Deconvolution, Image deconvolution, Biomedical optics

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Proceedings Article | 12 March 2024 Presentation + Paper

Real-time in-vivo volumetric quantitative phase imaging with a compact handheld probe

Zhe Guang, Paloma Casteleiro Costa, Zhenmin Li, Joshua Lerner, Francisco Robles

Proceedings Volume 12852, 1285206 (2024) https://doi.org/10.1117/12.3000269

KEYWORDS: Biological samples, Phase imaging, Light sources and illumination, In vivo imaging, Imaging systems, Biological imaging, Brain, Tumors, Real time imaging, Design

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Proceedings Article | 16 March 2023 Presentation + Paper

Quantitative oblique back-illumination microscopy with enhanced nuclear phase contrast using acetic acid

Zhe Guang, Amunet Jacobs, Paloma Casteleiro Costa, Caroline Filan, Francisco Robles

Proceedings Volume 12389, 1238909 (2023) https://doi.org/10.1117/12.2650837

KEYWORDS: Microscopy, Biological samples, Phase contrast, Tissues, Brain, Phase imaging, Scattering, In vivo imaging, Confocal microscopy, Refractive index

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Proceedings Article | 16 March 2023 Presentation + Paper

Single capture quantitative phase imaging with tomographic sectioning

Paloma Casteleiro Costa, Zhe Guang, Nischita Kaza, Francisco Robles

Proceedings Volume 12389, 1238906 (2023) https://doi.org/10.1117/12.2650539

KEYWORDS: Image restoration, Phase imaging, Biological samples, Tomography, Brain, Blood, Microscopy, Phase reconstruction, Tissues

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Proceedings Article | 16 March 2023 Presentation + Paper

Handheld quantitative phase imaging probe for in-vivo brain tumor margin assessment

Zhe Guang, Paloma Casteleiro Costa, Amunet Jacobs, Caroline Filan, Francisco Robles

Proceedings Volume 12391, 123910C (2023) https://doi.org/10.1117/12.2650851

KEYWORDS: Tumors, Brain, Phase imaging, In vivo imaging, Biological samples

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Proceedings Article | 1 April 2022 Presentation

Development and experimental validation of rapid retrieval algorithm of first-order spatiotemporal distortions for ultrashort laser pulses

Elizabeth Grace, Tammy Ma, Zhe Guang, Raspberry Simpson, Graeme Scott, Derek Mariscal, Brent Stuart, Rick Trebino

Proceedings Volume PC11986, PC1198602 (2022) https://doi.org/10.1117/12.2607419

KEYWORDS: Algorithm development, Ultrafast phenomena, Laser development, Diagnostics, Spatial resolution, Pulsed laser operation, Physics, Holograms, Computer simulations

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SPIE Journal Paper | 14 July 2021 Open Access

Label-free detection of brain tumors in a 9L gliosarcoma rat model using stimulated Raman scattering-spectroscopic optical coherence tomography

Soheil Soltani, Zhe Guang, Zhaobin Zhang, Jeffrey Olson, Francisco Robles

JBO, Vol. 26, Issue 07, 076004, (July 2021) https://doi.org/10.1117/12.10.1117/1.JBO.26.7.076004

KEYWORDS: Tumors, Brain, Optical coherence tomography, Tissues, Raman spectroscopy, Proteins, Signal attenuation, Neuroimaging, Signal detection, Nonlinear optics

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Significance: In neurosurgery, it is essential to differentiate between tumor and healthy brain regions to maximize tumor resection while minimizing damage to vital healthy brain tissue. However, conventional intraoperative imaging tools used to guide neurosurgery are often unable to distinguish tumor margins, particularly in infiltrative tumor regions and low-grade gliomas. Aim: The aim of this work is to assess the feasibility of a label-free molecular imaging tool called stimulated Raman scattering-spectroscopic optical coherence tomography (SRS-SOCT) to differentiate between healthy brain tissue and tumor based on (1) structural biomarkers derived from the decay rate of signals as a function of depth and (2) molecular biomarkers based on relative differences in lipid and protein composition extracted from the SRS signals. Approach: SRS-SOCT combines the molecular sensitivity of SRS (based on vibrational spectroscopy) with the spatial and spectral multiplexing capabilities of SOCT to enable fast, spatially and spectrally resolved molecular imaging. SRS-SOCT is applied to image a 9L gliosarcoma rat tumor model, a well-characterized model that recapitulates human high-grade gliomas, including high proliferative capability, high vascularization, and infiltration at the margin. Structural and biochemical signatures acquired from SRS-SOCT are extracted to identify healthy and tumor tissues. Results: Data show that SRS-SOCT provides light-scattering-based signatures that correlate with the presence of tumors, similar to conventional OCT. Further, nonlinear phase changes from the SRS interaction, as measured with SRS-SOCT, provide an additional measure to clearly separate tumor tissue from healthy brain regions. We also show that the nonlinear phase signals in SRS-SOCT provide a signal-to-noise advantage over the nonlinear amplitude signals for identifying tumors. Conclusions: SRS-SOCT can distinguish both spatial and spectral features that identify tumor regions in the 9L gliosarcoma rat model. This tool provides fast, label-free, nondestructive, and spatially resolved molecular information that, with future development, can potentially assist in identifying tumor margins in neurosurgery.

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Proceedings Article | 5 March 2021 Presentation

Complete single-shot spatiotemporal measurement of a terawatt laser pulse

Elizabeth Grace, Tammy Ma, Zhe Guang, Rana Jafari, Jaebum Park, Jerry Clark, Gregory Kemp, James Moody, Ronnie Shepherd, Brent Stuart, Rick Trebino

Proceedings Volume 11684, 1168406 (2021) https://doi.org/10.1117/12.2578720

KEYWORDS: Pulsed laser operation, Measurement devices, Laser systems engineering, Optical filters, Holograms, Diagnostics, Comets, Beam splitters

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Proceedings Article | 5 March 2021 Poster + Presentation + Paper

Quantitative phase imaging of thick scattering samples through a flexible fiber-optic bundle

Zhe Guang, Patrick Ledwig, Paloma Casteleiro Costa, Francisco Robles

Proceedings Volume 11653, 116531G (2021) https://doi.org/10.1117/12.2576688

KEYWORDS: Scattering, Phase imaging, Fiber optics, Tissues, Photons, Imaging systems, Biomedical optics, Tumors, Statistical analysis, Optical fibers

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Proceedings Article | 5 March 2021 Presentation + Paper

Epi-illumination tomographic quantitative phase imaging identifies brain tumor margins

Paloma Casteleiro Costa, Zhe Guang, Patrick Ledwig, Zhaobin Zhang, Stewart Neill, Jeffrey Olson, Francisco Robles

Proceedings Volume 11655, 116550S (2021) https://doi.org/10.1117/12.2577216

KEYWORDS: Tumors, Brain, Tomography, Phase imaging, Neuroimaging, Tissues, Surgery, Tissue optics, Systems modeling, Standards development

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Proceedings Article | 14 February 2020 Paper

Quantitative phase imaging with epi-mode illumination for fiber-optic endoscopy

Zhe Guang, Patrick Ledwig, Paloma Casteleiro Costa, Francisco Robles

Proceedings Volume 11249, 112491T (2020) https://doi.org/10.1117/12.2546402

KEYWORDS: Endoscopy, Fiber optics, Microscopy, Phase imaging, Phase retrieval, Brain, Scattering, Light emitting diodes, Tissues, Biomedical optics

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Proceedings Article | 22 February 2017 Presentation + Paper

Real-time spatiotemporal measurement of ultrafast fields from multimode optical fibers

Zhe Guang, Michelle Rhodes, Ping Zhu, Rick Trebino

Proceedings Volume 10089, 100890B (2017) https://doi.org/10.1117/12.2255637

KEYWORDS: Ultrafast phenomena, Multimode fibers, Optical fibers, Holograms, Ultrafast measurement systems, Cameras, Digital holography, Pulsed laser operation, Second-harmonic generation, Physics

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Proceedings Article | 22 February 2017 Presentation + Paper

Spatiotemporal coupling effects in ultrashort pulses and their visualization

Michelle Rhodes, Zhe Guang, Rick Trebino

Proceedings Volume 10089, 100890I (2017) https://doi.org/10.1117/12.2251632

KEYWORDS: Ultrafast phenomena, Visualization, Laser development, Diffraction, Singular optics, Temporal resolution, RGB color model, Fourier transforms, Spectral resolution, Radio propagation, Wavefronts, Near field

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Proceedings Article | 9 March 2016 Paper

Measuring spatiotemporal intensity-and-phase complexity of multimode fiber output pulses

Zhe Guang, Michelle Rhodes, Rick Trebino

Proceedings Volume 9740, 97400G (2016) https://doi.org/10.1117/12.2211912

KEYWORDS: Ultrafast phenomena, Multimode fibers, Wave propagation, Fiber characterization, Telecommunications, Beam propagation method, Fiber optics tests, Fiber optics, Single mode fibers, Data communications, Holograms, Cameras, Structured optical fibers, Optical testing, Optical fibers, Sapphire lasers

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Proceedings Article | 9 March 2016 Paper

Unstable multipulsing can be invisible to some ultrashort pulse measurement techniques

Michelle Rhodes, Zhe Guang, Rick Trebino

Proceedings Volume 9732, 97320R (2016) https://doi.org/10.1117/12.2213131

KEYWORDS: Satellites, Ultrafast phenomena, Mode locking, Ultrafast lasers, Laser systems engineering, Interferometry, Visible radiation, Neodymium, Modulation, Second-harmonic generation, Fiber lasers, Phase shift keying

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Proceedings Article | 9 March 2015 Paper

Simple single-shot complete spatiotemporal characterization of the intensity and phase of a complex ultrashort pulse

Zhe Guang, Michelle Rhodes, Rick Trebino

Proceedings Volume 9355, 93550J (2015) https://doi.org/10.1117/12.2077766

KEYWORDS: Holograms, Cameras, Ultrafast phenomena, Digital holography, Diffractive optical elements, Phase measurement, Photography, Optical filters, Oscillators, Photographic lenses

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Showing 5 of 19 publications

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