Plasmodium falciparum and Plasmodium vivax species are associated for malarial infections primarily in south-African and south-Asian sub-continents respectively. In this work we extract the refractive index of whole blood (WB) infected with Plasmodium falciparum or Plasmodium vivax species. Surface plasmon microscopy is used for the extraction of refractive index of WB. We conclude that intrinsic physical parameters, such as refractive index, can be used as a quantitative label-free estimator for the detection of different plasmodium species with varying densities in human WB.
Circadian rhythm exerts a critical role for the determination of health and consequently diseases. We investigate the dependence of this parameter non-invasively by measuring the refractive index of saliva from human beings. The refractive index measurement is conducted by a probe developed with focused surface plasmons.
In this proceeding, we search an optimized substrate for live cell imaging in culture medium with surface plasmon microscopy with high sensitivity. Coverslips coated with nano-metric bimetallic gold and silver best serves the purpose.
In this proceeding, we characterize the anisotropic properties of skin that strongly scatter propagating light. We conduct the measurement by probing the optical near field from the sample side with focused surface plasmon.
In this proceeding, we study very low birefringence changes in microscopic sites from the spatial frequency response of focused surface plasmon. For the feasibility test, we consider thin films of photo-addressable azo-polymers as potential samples. We confirmed the proposition with the help of various concentrations of thin films of azo-polymers resulting in various orders of photo-induced birefringence even as low as 0.01. Further, we test human dermal fibroblasts (HDFs) birefringence changes in various environments towards label free detection of hotspots of dermatoheliosis.
In this paper we analyze the relationship between the spatial frequency response of focused surface plasmon with the birefringence of the sample. The sample consists of thin film of photo-addressable azobenzene functionalized homopolymer. Due to the photoisomerization of the cis-trans relaxation states of azopolymer, anisotropy is induced in the sample and absorption pattern with ellipticity of 1.082 is observed at the exit pupil plane. Quantitative analysis of the ellipticity and orientation of the absorption pattern reveals the magnitude of birefringence and direction of fast axis of the sample. Simulated and experimental results are presented.
In this paper, we report an interferometric method to extract the phase information available in a desired band of spatial frequencies. The phase sample is placed in the path of a converging beam of light entering a Mach Zehnder interferometer so that the two Fourier Transform (FT) planes are located in the two interferometer arms. One of the FT planes is filtered by suitable masks so that only the frequencies that are blocked appears in the final interferogram. An imaging lens images the phase object on a CCD. Polarization Phase Shifting is incorporated so that the final frequency filtered image is reconstructed from the four phase shifted interferograms. The interferometer is made to operate in null fringe condition so that residual phase is eliminated. Simulated and experimental results are presented.
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