Low- and high-dose laser irradiation effects on cell migration and destruction

Elivia Layton; Kyra A. Gallagher; Sara Zukerman; Brianna Stevens; Feifan Zhou; Hong Liu; Wei R. Chen

doi:10.1117/12.2290654

21 February 2018 Low- and high-dose laser irradiation effects on cell migration and destruction

Elivia Layton, Kyra A. Gallagher, Sara Zukerman, Brianna Stevens, Feifan Zhou, Hong Liu, Wei R. Chen

Proceedings Volume 10495, Biophotonics and Immune Responses XIII; 1049515 (2018) https://doi.org/10.1117/12.2290654
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Metastases are the cause of more than 90 percent of cancer-related deaths. Current treatment methods, including chemotherapy, radiation, and surgery, fail to target the metastases effectively. One potential treatment for metastatic cancer is laser immunotherapy (LIT). LIT combines the use of a photothermal laser with an immunoadjuvant, Glycated Chitosan (GC). GC combined with single-walled carbon nanotubes (SWNTs) has proven to be a viable alternative to traditional cancer treatment methods, when under irradiation of laser with appropriate wavelength. In this study, the effects of low dose and high dose laser irradiation on metastatic pancreatic cancer cell migration were observed. It was found that low dose irradiation increased the migration rate, but the high dose irradiation significantly decreased the migration rate of the cancer cells. When using LIT, the goal is to kill tumor cells and to prompt the correct immune response. If the tumor were irradiated with a low dose, it would promote metastasis. If the dose of irradiation were too high, it would destroy the entire tumor and the immune response would not recognize the tumor. Therefore, the laser dose plays an important role in LIT, particularly when using SWNT as light absorbing agent. Our results from this study will delineate the optimal laser irradiation dose for destroying tumor cells and at the same time preserve and release tumor antigens as a precursor of antitumor immune response.

Citation Download Citation

Elivia Layton, Kyra A. Gallagher, Sara Zukerman, Brianna Stevens, Feifan Zhou, Hong Liu, and Wei R. Chen "Low- and high-dose laser irradiation effects on cell migration and destruction", Proc. SPIE 10495, Biophotonics and Immune Responses XIII, 1049515 (21 February 2018); https://doi.org/10.1117/12.2290654

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