The effects of laser immunotherapy on cancer cell migration

Cody F. Bahavar; Feifan Zhou; Aamr M. Hasanjee; Elivia Layton; Anh Lam; Wei R. Chen; Melville B. Vaughan

doi:10.1117/12.2216592

10 March 2016 The effects of laser immunotherapy on cancer cell migration

Cody F. Bahavar, Feifan Zhou, Aamr M. Hasanjee, Elivia Layton, Anh Lam, Wei R. Chen, Melville B. Vaughan

Proceedings Volume 9709, Biophotonics and Immune Responses XI; 97090X (2016) https://doi.org/10.1117/12.2216592
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Laser immunotherapy (LIT) uses laser irradiation and immunological stimulation to target all types of metastases and creates a long-term tumor resistance. Glycated chitosan (GC) is the immunological stimulant used in LIT. Interestingly, GC can act as a surfactant for single-walled carbon nanotubes (SWNTs) to immunologically modify SWNTs. SWNT-GC retains the optical properties of SWNTs and the immunological functions of GC to help increase the selectivity of the laser and create a more optimal immune response. One essential aspect of understanding this immune response is knowing how laser irradiation affects cancer cells’ ability to metastasize. In this experiment, a cell migration assay was performed. A 2mm circular elastomer plugs were placed at the bottom of multi-well dishes. Pre-cancerous keratinocytes, different tumor cells, and fibroblasts were then plated separately in treated wells. Once the cells reached 100% confluence, they were irradiated by either a 980nm or 805nm wavelength laser. The goal was to determine the effects of laser irradiation and immunological stimulation on cancer cell migration in vitro, paying the way to understand the mechanism of LIT in treating metastatic tumors in cancer patients.

Citation Download Citation

Cody F. Bahavar, Feifan Zhou, Aamr M. Hasanjee, Elivia Layton, Anh Lam, Wei R. Chen, and Melville B. Vaughan "The effects of laser immunotherapy on cancer cell migration", Proc. SPIE 9709, Biophotonics and Immune Responses XI, 97090X (10 March 2016); https://doi.org/10.1117/12.2216592

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KEYWORDS

Cancer

Single walled carbon nanotubes

Tumors

Laser irradiation

Biomedical optics

In vitro testing

Optical properties

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