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12 March 2019 Two-photon laser scanning microscopy integrated with light stimulus system
Xin Xu, Xingjie Jin, Feng Gao, Guohua Shi
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Proceedings Volume 11023, Fifth Symposium on Novel Optoelectronic Detection Technology and Application; 1102346 (2019) https://doi.org/10.1117/12.2521012
Event: Fifth Symposium on Novel Optoelectronic Detection Technology and Application, 2018, Xi'an, China
Abstract
We demonstrate a home-made two-photon laser scanning microscopy (TPLSM) with a light stimulus system. In this system, the femtosecond pulses are produced by a picosecond fiber laser with pulse width compression. A laser diode serves as stimulus, which modulated by an AOM and coupled into the same light path of a femtosecond laser. The control signal of AOM and trigger signal of two scanning mirrors are synchronized by a FPGA board. With modulating the intensity of CW laser at precise point in one scanning frame, any pattern of light stimulus can be delivered to the sample in real time.
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Xin Xu, Xingjie Jin, Feng Gao, and Guohua Shi "Two-photon laser scanning microscopy integrated with light stimulus system", Proc. SPIE 11023, Fifth Symposium on Novel Optoelectronic Detection Technology and Application, 1102346 (12 March 2019); https://doi.org/10.1117/12.2521012
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KEYWORDS
Femtosecond phenomena
Modulation
Microscopy
Two photon imaging
Field programmable gate arrays
Laser scanners
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