High-speed imaging of cilia beat frequency using phase-resolved spectrally encoded interferometric microscopy

Do A. Kim; Zhikai Zhu; Lidek Chou; Katelyn Dilley; Theodore V, Nguyen; Edward Kuan; Brian Wong; Zhongping Chen

doi:10.1117/12.2651305

14 March 2023 High-speed imaging of cilia beat frequency using phase-resolved spectrally encoded interferometric microscopy

Do A. Kim, Zhikai Zhu, Lidek Chou, Katelyn Dilley, Theodore V, Nguyen, Edward Kuan, Brian Wong, Zhongping Chen

Author Affiliations +

Proceedings Volume 12354, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2023; 1235407 (2023) https://doi.org/10.1117/12.2651305
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mucociliary clearance is vital for preventing any foreign substances from entering the upper airway that can later develop into acute and/or chronic respiratory diseases. Therefore, it is essential to further advance our understanding of the mucociliary functions. Our lab has been able to make key developments in imaging cilia, specifically measuring cilia beat frequency, with phase-resolved Doppler optical coherence tomography. In this system, we have further developed the system by incorporating phase-resolved spectrally encoded interferometric microscopy (SIEM) system with an FDML laser with MHz sweep rate to image cilia with higher accuracy and to minimize motion artifacts. In addition, we have designed a compact handheld probe system with a GRIN lens for easier in vivo imaging. The development of this system will allow us to further investigate cilia dynamics and ultimately utilize the system for clinical applications.

Conference Presentation

Citation Download Citation

Do A. Kim, Zhikai Zhu, Lidek Chou, Katelyn Dilley, Theodore V, Nguyen, Edward Kuan, Brian Wong, and Zhongping Chen "High-speed imaging of cilia beat frequency using phase-resolved spectrally encoded interferometric microscopy", Proc. SPIE 12354, Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2023, 1235407 (14 March 2023); https://doi.org/10.1117/12.2651305

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