Improved multiwavelength twin image and coherent noise reduction in Gabor holographic microscopy

Maciej Trusiak; Mikolaj Rogalski

doi:10.1117/12.2673882

12 August 2023 Improved multiwavelength twin image and coherent noise reduction in Gabor holographic microscopy

Maciej Trusiak, Mikolaj Rogalski

Proceedings Volume PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; PC1262206 (2023) https://doi.org/10.1117/12.2673882
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

A normal bright-field microscope may be updated with coherent sensing capabilities easily, inexpensively, and compactly using phase imaging microscopy in the Gabor regime. The digital sensor records an in-line Gabor hologram of the target sample by incorporating coherent illumination into the regular microscope embodiment, generated by a small defocus distance of the sample at the input plane. This hologram is then numerically post-processed to obtain the quantitative phase information of the sample. However, when dealing with Gabor's regime, coherent noise and twin-image disturbances affect the recovered phase distribution. In this contribution, we describe a single-shot method for reducing these two error sources based on wavelength multiplexing. The sample is illuminated by a multi-wavelength laser source that utilized three diode lasers, and the wavelength-multiplexed Gabor hologram is to be captured on a digital color sensor using a traditional RGB color camera in a single exposure. The presented phase imaging method is completed by the implementation of a new algorithm built on a modified Gerchberg-Saxton kernel to obtain an enhanced quantitative phase image of a sample that has been improved in terms of coherent noise removal and twin-image reduction. Complex field filtering in terms of hologram's imaginary and real part numerical alteration is in place in an iterative fashion. Experimental validations are carried out in a off-the-shelf Olympus BX-60 upright microscope with a 20X 0.46NA objective lens employing static (resolution test targets) and dynamic (live spermatozoa) phase sample quantitative imaging.

Conference Presentation

Citation Download Citation

Maciej Trusiak and Mikolaj Rogalski "Improved multiwavelength twin image and coherent noise reduction in Gabor holographic microscopy", Proc. SPIE PC12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, PC1262206 (12 August 2023); https://doi.org/10.1117/12.2673882

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