We present a novel implementation of high-speed photoacoustic microscopy with an ultrawide field of view (FOV). Existing photoacoustic microscopy systems have limitations in resolution, FOV, and speed. To address these challenges, we improved our previous immersible-polygon-scanner-based photoacoustic microscopy and developed a dual-view photoacoustic microscopy system using two facets of the same scanner. The system has achieved high resolutions of ~10 µm (lateral) and 35 µm (axial) with a B-scan rate of 500 Hz. We have demonstrated the imaging performance on freely-moving zebrafish and hypoxia challenge of a pair of mice.
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