Mr. Roberto Pilloton Profile

Roberto Pilloton

at ENEA

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Proceedings Article | 7 January 2009 Paper

Developing a miniaturized continuous flow electrochemical cell for biosensor applications

M. Ilie, E. Ovreiu, R. Dejana, V. Foglietti, L. Nardi, A. Masci, B. Lanza, L. Della Seta, M-R. Montereali, W. Vastarella, R. Pilloton

Proceedings Volume 7297, 729724 (2009) https://doi.org/10.1117/12.823688

KEYWORDS: Electrodes, Glasses, Biosensors, Microfluidics, Silicon, Signal processing, Gold, Borosilicate glass, Connectors, Reliability

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The development of a miniaturized electrochemical cell for biosensor application regards both the structuring of an array of electrodes in a fluidic chamber and their connections to the control & processing unit The sensitivity of the chrono-amperometric measurement performed with the cell is increased by: (a) integrating the reference electrode on the same chip with the counter- and working- electrodes, (b) designing a specific pattern of the gold electrodes and (c) serially distributing them along the pipeline reservoir. Borosilicate glass is used as substrate for the electrodes, allowing, due to its transparency, an accurate and easy pad to pad alignment of the up-side-down chip versus a PCB soldered on a standard DIL 40 socket. This alignment is necessary to accomplish the elastomer-based-solderless electric contact, between chip and PCB. The solderless contact significantly improves both reliability and signal processing accuracy. The reservoir and its cover are micromachined out of silicone rubber respectively photosensitive glass in order to easy disassemble the fluidic chamber without any damage. Both thickness and elasticity of the photosensitive glass rend the device less brittle. A plug-in -plug-flow device with improved characteristics has been obtained with a modular structure that allows further extension of the number of electrodes.

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